Methods: The effects of shikonin on proliferation and apoptosis of colo-16 cell line were assessed using culture technique of colo-16 in vitro, MTT, fluorescence testing and flow cyctometry assay.

• 方法 : 利用体外细胞培养技术、MTT法、流式细胞仪、荧光显微镜技术检测不同浓度的紫草素对colo- 16细胞株增殖及凋亡的影响。