Methods: The gene fragment encoding the Z domain of Protein A(Protein A-Z) was generated by Overlap PCR amplification, and was cloned into TA-cloning vector pMD-18T.

• 方法:应用Overlap PCR的方法制备Protein A的Z结构域(Protein A-Z),克隆于pMD-18T载体构建PA-Z/pMD-18T重组质粒。