Methods: male Wistar rats were injected Azaser-ine 30mg/kg(ip). Some rats received Estradiol-17 P Valerate(0.18mg/100g/2weeks, im) .

• 方法 雄性Wistar大鼠单次腹腔注射重氮乙酰丝氨酸30mg/kg；部分大鼠2个月后开始肌肉汪射戊酸雌二醇0.；18mg/100g×2周；4个月后；常规取胰腺组织行光镜和电镜检查；并应用PCR-SSCP法检测K-ras基因突变情况