Methods:A plasmid including U6 promoter and siRNA of hTERT was designed and constructed. The plasmid was transfected into ALVA-41 cell line. The telomerase activity was tested by telomerase repeat amplification protocol ELISA(TRAP-ELISA).

• 方法:设计和构建由U6启动子驱动产生hTERT的siRNA表达质粒,转染至ALVA-41前列腺肿瘤细胞,以TRAP-ELISA方法观察细胞端粒酶活性,W estern-b lot检测端粒酶蛋白的表达,细胞记数法检测对细胞生长的影响。