Methods:Gradient PCR was employed in analysis of aldosterone synthase(CYP11B2)gene polymorphisms,and gel Imaging Technology was used to determine the optimal annealing temperature.

• 方法:采用梯度PCR对醛固酮合酶基因(CYP11B2)进行扩增,通过凝胶呈像技术判断CYP11B2基因最佳退火温度。