Methods:LPS induced iNOS mRNA expression, production of NO and effects of activated macrophages on anti tumor were investigated separately by RT PCR, nitrate reductase method and 3HTdR incorporation.
英
美
- 方法 :应用RT PCR、硝酸还原酶法、[3H]TdR掺入法分别观察LPS诱导的巨噬细胞iNOSmRNA表达、NO产生以及巨噬细胞抗肿瘤细胞毒作用。