Methods:The fermentation extracts were isolated and purified from endophyte HCCB00189 by solvent extraction,column chromatography and preparative HPLC,respectively.
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- 方法:采用溶剂萃取、硅胶柱层析及制备HPLC等方法,对活性菌株的发酵物进行分离,并根据理化性质和光谱分析对化学结构进行鉴定。