Methods:The full-length gene of NY-ESO-1 was generated by gene splicing method and the recombinant expression vector NY-ESO-1-pET-28a (+) was constructed. E. coli BL21 (DE3) bearing the plasmid was induced with IPTG for protein production.

• 方法:通过全基因拼接获得NY-ESO-1基因,构建重组表达载体NY-ESO-1-pET28a(+),在大肠杆菌BL21(DE3)中利用IPTG诱导获得表达,利用单克隆抗体进行Western印迹鉴定,通过Ni柱亲和纯化获得纯化蛋白。