Methods:The proteins of HeLa cell were extracted by three different lysis buffers to do 2-DE. The gel images then were acquired by Amersham ImageMaster VDS-CL to do the comparative analysis with PDQuest(7.4.0)software.

• 方法:用三种不同配方的裂解液提取HeLa细胞中的蛋白质；进行双向凝胶电泳；用Amersham ImageMaster VDS-CL型凝胶成像系统获取凝胶图像；以PDQuest(7.；4