Mice were inoculated with the vaccine and specific cellular and humoral immunity against flk1 were studied by 4 h 51Cr release test and immunochemistry method.

• 使用疫苗肌肉注射免疫小鼠，使用免疫小鼠脾细胞作为效应细胞，使用处于增殖期的小鼠内皮细胞作为靶细胞，标准4 h 51Cr释放试验检验特异性CTL反应，免疫组化法检测血清中特异性抗体的存在；