Mobile phase 10g/L KH 2PO 4 (pH 3.5): ACN is 70:30.The speed of mobile phase was 1.0ml/min.

• 色谱柱为InertsilODS - 3；柱温为室温 ；流动相为磷酸二氢钠 (10g/l磷酸调pH 3.；5 ) :甲醇 =70 :30；流速为 1