Mouse GM-CSF and B7.1 cDNA were spliced through a linker sequence byover-lap extention PCR and the mGM-B7.1 fusion gene was also ligated into theretrovirus plasmid pLXSN and its sequence was analyzed.

• 通过 Lipofectamine 2000 介导的转染法将逆转录病毒载体 pLXSN 及构建于逆转录病毒载体上的小鼠 GM-CSF、B7.；1 基因 cDNA 和mGM-B7