Myoblasts were obtained from newborn mice skeletal muscle and grown in DMEM/F12 supplied with 10% FBS for 36-48 hours,and then exposed to 0.5% FBS medium with or without macrophage-conditioned medium.
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- 取生后5d小鼠的成肌细胞;在含10%25血清的DMEM/F12培养液中培养36-48h后;将血清浓度降低至0.;5%25。