Object: Set up a method of isolation and purification of early pregnancy factor (EPF) from human pregnancy sera and get pure EPF, then attempt to prepare monoclonal antibodies.

• 目的:建立一条从孕早期妇女血清中分离纯化早孕因子(EPF)的技术路线,获得高纯度的EPF,并对制备抗EPF单克隆抗体进行阶段性的初步尝试。