Objective This study aimed to establish a fast and accuratequantitative method to detect Borna disease virus (BDV) ribonucleic acid(RNA) using fluorescence quantitative nested reverse transcriptasepolymerase chain reaction (FQ-nRT-PCR).

• 目的：本研究拟建立博尔纳病病毒(BDV)荧光定量套式逆转录酶聚合酶链反应(FQ-nRT-PCR)检测方法，为快速、准确地定量检测 BDV奠定基础。