Objective To construct spinocerebellar ataxia 3 (SCA3) eukarotic expression vector with enhanced green fluorescent protein (EGFP) as report gene and to observe its expression in PC12 cells.

• 目的构建以增强型绿色荧光蛋白(EGFP)作为报告基因、带有脊髓小脑性共济失调3型(SCA3)基因的真核细胞表达载体,并转染PC12细胞,观察外源基因的表达情况。