Objective To establish an immunoblotting test(IBT)for detecting the antibodies of extractable nuclear antigen(ENA)with rabbit thymus acetone powder made by ourselves. Methods The thymus of rabbits was separated to extract the ENA antigens.

• 目的 自制兔胸腺可提取性核抗原 (ENA)多肽 ,应用免疫印迹技术 (IBT)建立检测ENA多肽抗体的方法。