Objective To establish anti-CD3 monoclonal antibody activated killer cells(CD3AK) culture system from 20 mL peripheral blood and observe the cytotoxity against tumor cells in vitro.

• 目的:建立从20mL外周血中高效扩增抗CD3单克隆抗体激活的杀伤细胞(CD3AK)的培养体系,并进行体外细胞毒活性检测。