Objective: Get the pure EPF from the pregnancy sera through the ion exchange chromatography, affinity chromatography and reverse phage High Performance Liquid Chromatography(HPLC).

• 目的：采用离子交换层析、亲和层析和反相高效液相色谱(HPLC，High Performance Liquid Chromatography)技术，从人早孕血清中分离纯化早孕因子(EPF，Early Pregnancy Factor)。