One is the flavin adenine dinucleotide (FAD), and the second is methenyltetrahydropteroylployglutamate (MTHF).

• 大肠杆菌photolyase含有两个辅基，分别为黄素腺嘌呤二核苷酸(FAD)和次甲基四氢叶酸(MTHF)。