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- A and B fragments of CYP2J3 gene were connected by overlap extension PCR to obtain ORF sequence of CYP2J3 gene. 运用重叠延伸聚合酶链反应连接CYP2J3基因A和B两片段,扩增出大鼠CYP2J3基因全长ORF序列。
- Hyperparasitic effect was observed at culture over lap of Cylindrocladium spathiphylli and some phytopathogen through microscope. 显微观察对峙培养的重叠区域，发现苞叶芋柱帚霉对部分病原菌有重寄生现象。
- In order to improve the optimum temperature of lipases, The Penidilum expansuin lipase (PEL) gene was mutated by site-directed mutagenesis using overlap extension PCR technique. 利用重叠延伸PCR法对扩展青霉碱性脂肪酶(PEL)基因作了体外定点突变，获得了最适作用温度有所提高的突变体。
- Methods The Ecs gene was cloned using overlap extension PCR and the condon of Met28 was mutated to Leu. Three pieces of Ecs genes were linked in the same orientation and inserted into expression vector pET30a. The recombinant plasmid was expressed in E. 方法:采用重叠延伸PCR克隆Ecs基因;将第28位Met的密码子突变为Leu;利用其序列特点及酶切位点;在表达载体pET30a上将Ecs基因以同向串联方式连接;在E.
- In this paper, mechanical analysis of over lap section of yieldable steel ribs is made.It describes how to deal with the overlap sections in calculation of internal forces in the support. 本文根据地下结构的特点，着重对可缩性金属支架的搭接段进行力学分析，并提出了在支架内力计算中搭接段的处理方法；
- splicing by overlap extension PCR 重叠延伸PCR（SOEPCR）
- gene splicing by overlap extension PCR 重叠延伸PCR
- Over lap PCR 重叠PCR
- He tried to varnish over the truth with a lie. 他试图用谎言来掩盖真相。
- I've got the hump over what he said to me. 我已经对他所说的很不耐烦了。
- She threw a scarf over her shoulders. 她在肩上披上一块肩巾。
- The boat heeled over in a strong wind. 船在强风吹袭下倾侧了。
- Blind FRESH (frequency shift filter) can separate desired signals from spectrally over lapping interference by exploiting cyclostationarity. 摘要利用循环平稳特性可以进行信号提取，频移滤波器对循环频率误差较为敏感。
- They all cooed over the new baby. 他们对着新生婴儿爱抚地轻声细语。
- The dog kept a vigilant guard over the house. 这只狗警惕地守护着这所房屋。
- Put the slats on the roof so that they lap over. 把石板瓦安放在屋顶上，使之相互叠盖。
- She worried over her father's health. 她为父亲的健康担忧。
- The two pictures are made to lap over each other . 那两张画被重叠在一起。
- The brook warbled over its rocky bed. 小溪潺潺流过多石的河床。
- I fell off my bike and went arse over tit. 我跌下自行车，一头号栽到地上。
