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- Less-than-one-hour in situ hybridization manual met hod for immunocytochemical analyses of formalin-fixed, paraffin embedded tissu e sections was used to detect infectious agents. 快速原位杂交技术是一种近年才发展起来的检测常规病理标本中有无感染原存在的非常有效的工具，与现在普遍采用的原位杂交技术相比具有更强的核酸定位能力。
- Methods The paraffin embedded tissue sections from 33 patients with TCC of bladder were reexamined. According to the UICC standard, 21 cases had surperficial tumors and 12 cases had invasive tumors. 方法 33例膀胱 TCC石蜡块标本均经病理复检 ,12例正常膀胱组织作为对照组。
- Extraction of DNA from formalin fixed and paraffin embedded tissues and its effect on PCR 福尔马林固定石蜡包埋组织DNA提取方法对pcr的影响
- Detection of Helicobacter Pylori in Paraffin Embedded Tissues with Polymerase Chain Reaction for Patients with Gastric Dis-eases 聚合酶链反应检测胃部疾患石蜡包埋组织中的幽门螺杆菌
- Detection of EWS-FLI1 fusion transcripts in paraffin embedded tissues of peripheral primitive neuroectodermal tumors by nested reverse transcription polymerase chain reaction RT-PCR法检测外周原始神经外胚叶瘤石蜡包埋组织中EWS-FLI1融合基因
- Keywords formalin fixed;paraffin embedded tissue;DNA extraction; 关键词福尔马林固定;石蜡包埋组织;DNA提取;
- Methods:Overexpressions of p 53 and C erbB 2,C ras,C myc were determined by immunohistochemistry in paraffin embedded tumor tissues from 25 patients with GCT. 方法：采用单克隆抗体免疫组化技术，对25份卵巢粒层细胞瘤组织进行p53、C－erbB2、C－ras及C－myc表达的检测，分析基因表达与临床－病理指标及患者预后的关系。
- DNA content and cell proliferative activity in paraffin embedded tissue of mycosis fungoides 蕈样肉芽肿皮损中DNA含量及细胞增殖活性的检测
- Methods: ER and PR contents of fresh tumor tissues taken from 70 cases of primary endometrial carcinoma were measured by biochemical (DCC) method and 30 paraffin embedded archival specimens of the 70 cases also by immunohistochemical (IHC) method. 方法：用葡聚糖－活性碳吸附法（DCC法）对70例子宫内膜癌原发病灶组织进行ER及PR测定，同时用免疫组化法对其中30例的石蜡标本进行了ER及PR的检测。
- paraffin embedded tissue 石蜡包埋组织
- Conclusions ISPCR could be applicable in detecting the DNA of T.gondii in paraffin embedded sections as a new method. 直接法原位PCR为检测病理标本中的弓形虫DNA提供了一种新的可行方法。
- METHODS:Beecher tissue arrayer was used to create a 196 dot tissue microarray, derived from 99 cases of common carcinomas, and their para cancerous tissues and corresponding normal tissues which were formalin fixed and paraffin embedded. 方法 :在病理档案材料中选取 10种常见恶性肿瘤及其癌旁或正常组织的石蜡包埋标本 ,HE染色切片定位穿刺部位 ,每一蜡块穿取直径 0 6mm大小组织柱 ,排布成 196点列阵的芯片 ;
- METHODS VEGF expression was detected in 64 paraffin embedded hepatic hemangioma slides with immunohistochemical staining (SABC) and AgNOR counting. 方法 用链霉亲和素 -生物素 -过氧化物酶免疫组织化学染色 (SABC法 )和核仁组成区相关嗜银蛋白 (Ag NOR)计数方法 ;研究 6 4例肝血管瘤标本 .
- Methods 42 paraffin embedded specimens of BCC were identified under light microscopy. P53 protein expression was observed using S P immunohistochemistry. 2方法 42例经常规石蜡包埋皮肤基底细胞癌标本 ,镜检确定组织学类型 ,应用免疫组织化学 S- P法检测 P5 3蛋白表达。
- Methods General paraffin embedded section and immunohistochemical method (ABC method) were taken in biopsy or surgical specimens of five CMALT oma and five CPL. 方法：CMALT－oma及CPL各5例，活检或手术标本做石蜡切片及免疫组化（ABC法）染色。
- Methods: The expression of FHIT was detected by SP immunohistochemical technique in 80 paraffin embedded specimens of HNSCC and 41 nomal squamous epithelium (NSE) specimens. 方法:应用免疫组化SP法,检测80例HNSCC及41例正常鳞状上皮标本中FHIT的表达。
- Methods 39 cases of CT SBB were studied by fast paraffin embedded sections, with comparison of further routine sections of the biopsy or/and the resected specimen. 方法39例CT引导立体定向脑活检术的标本,将其快速石蜡切片与其随后的常规切片或/和手术切除标本进行对比观察。
- Archival formalin fixed, paraffin embedded specimens from 55 primary NPC patients, who had biopsy in Cancer center, Sun Yat-sen University during April in 1996 to June in 1998, were investigated. 收集1996年4月至1998年6月在中山大学肿瘤防治中心鼻咽癌科行鼻咽活检的鼻咽癌患者的标本55份。
- Methods The CP embedding tissue section technique was used. 方法应用CP包埋组织切片技术。
- Methods E CD expression and cellular DNA content were quantitatively measured by flow cytometry and immunofluorescence methods in 80 cases of formalinfixed, paraffin embedded gastric cancer. 方法应用流式细胞术和细胞免疫荧光技术，定量检测了80例手术切除、石蜡包埋的胃癌标本中E－CD表达和DNA含量，并对切除胃标本做系统病理学检查。
