Primers,which were fit to CaMV35S,NOS,bar and tRNALeu gene,were chosen in the standards to detect and identify HWM transgenic wheat.The condition and system were optimized,and the electrophoresis result showed the expected strips.

• 针对该实验材料转入的外源基因选择了标准中通用的外源基因CaMV35S,NOS,bar和植物内源基因tRNALeu作为特异性引物,对影响定性PCR检测(反应体系:氯化镁浓度,引物和模板浓度;反应条件:退火温度和时间,循环数等)的主要条件进行了实验优化和选择,同时对PCR产物进行琼脂糖凝胶电泳分析。