Proliferation of splenocytes and thymocytes was measured by CFSE in response to in vitro T-cell receptor-dependent mitogens(ConA and anti-TCR),and to a cell surface-receptor-independent mitogenic combination(PMA+IONO) after cultured 72 h.

• CFSE标记后细胞 ,加入ConA、抗TCR抗体、PMA +IONO刺激剂培养 72小时 ,流式细胞仪分析的方法比较不同刺激剂作用下雌、雄小鼠T细胞的增殖。