Pure TS99 could be obtained from pretreatment fermentation broth through macroreticular adsorbent, silica gel column chromatography and thin-layer chromatography.

• 发酵液经过预处理后，依次用大孔树脂吸附，硅胶柱吸附层析和薄层层析等分离方法即可获得农用抗生素TS99纯品。