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- The expression of cyclin D1 and CDK4 were detected by RT PCR method. RT PCR检测细胞周期素D1(cyclinD1)、细胞周期蛋白依赖激酶 4 (CDK4 )mR NA的表达。
- Objective To construct and evaluate the competitive quantitative RT PCR method for detecting the fusion gene of AML1 ETO in the patients of t(8;21)AML(acute myeloid leukemia). 目的 建立针对 AML- ETO融合基因转录本的竞争性定量反向 -聚合酶链反应 (RT- PCR)体系 ,并探讨其在 t(8;2 1)急性髓细胞性白血病 (acute myeloid leukemia,AML)微小残留病变跟踪中的应用价值。
- Based on the human IL 15 cDNA sequence in GenBank,three primers of PCR were designed,and a RT PCR method of detecting human IL 15mRNA expression was developed. 本文依据CenBank人IL?15的cDNA序列,设计3个特异性引物,建立了检测IL?15基因表达的逆转录PCR(RT?PCR)方法。
- It is concordant with routine PCR method. 并与常规PCR法具有很好的一致性。
- The cDNA encoding the extracellular domain of human costimulatory molecule B7 1/CD80 was cloned from human peripheral blood monocytes by using RT PCR method and sequenced by Sanger chain termination method. 用RT PCR法从人外周血单核细胞克隆了编码共刺激分子B7 1 /CD80胞外区的cDNA ;并用Sanger链终止法进行测序 .
- The ISOObp promoter of PNZIP gene was cloned by adaptor PCR method. 因此,我们利用接头PCR技术克隆了PNZIP基因的启动子。
- The applications of Mullis' PCR method are already many. 原理简介:PCR技术的基本原理是DNA的半保留复制。
- Methods: The expression of Fas gene in 6 strains of ovarian carcinoma cells was detected with flow cytometry and RT PCR methods. 方法 :经流式细胞术、RT PCR方法检测 6种卵巢癌细胞Fas的表达水平。
- A multiplex- PCR method for detection bovine materials in foodstuffs. 动物饲料中牛成分的复合PCR检测方法。
- Meanwhile, the PCR method is superior to immunohistochemistry in detecting HPV. 在检测上,PCR方法优于免疫组织化学染色,值得提倡和推广。
- METHODS:The mRNA expression levels of IGF 1/IGF 1R were assessed by RT PCR method in the gastric cancer tissues, adjacent mucosa and tumor free tissues from 28 patients with gastric carcinoma and the normal gastric mucosa from 14 healthy controls. 方法 :对 2 8例经手术切除并由病理证实的胃癌患者的癌、癌旁及胃黏膜正常区组织标本进行研究 ,以 14例正常人胃黏膜标本作对照 ,应用RT PCR方法 ,检测IGF 1/IGF 1R在胃癌组织与正常人胃黏膜中的表达变化和胃癌患者的癌、癌旁及胃黏膜正常区组织中的表达变化。
- Methods: A cDNA fragment from the mRNAs of skeletal muscle of 13 day old chicken embryo was amplified by RT PCR method, and inserted into pGEM and pUC vectors at clone sites and sequenced respectively. 方法 :从 13d鸡胚骨骼肌mRNAs经RT PCR方法扩增RTEF 1的编码序列 (cDNA) ,将其克隆到 pGEM和 pUC载体并进行核苷酸序列分析。
- Using PCR method, BBTV or CMV can be detected from the banana leaf tissue equivalent to 100 ng. 用PCR方法可从相当于100ng的叶片组织中检测到BBTV和CMV。
- Objective To develop a PCR method for the determination of porcine parvovirus(PPV). 目的建立猪细小病毒(PPV)PCR检测方法。
- The results prove that adapter ligation PCR method is workable to clone carnation CHS promoter. 然后利用衔接头PCR的方法进行了香石竹CHS启动子克隆的初步研究,得到了约500bp和800bp的PCR产物,目前克隆测序工作正在进行。
- This is to develop a rapid,sensitive and specific fluorescence PCR method detecting listeria monocytogenes in food. 发展一种快速、敏感、特异的荧光PCR方法检测食品中单核细胞增生李斯特氏菌。
- In order to improve diagmostic procedure of E. suis, the PCR method was established. 鉴于上述原因,为了建立特异准确的诊断方法,本研究开展了附红细胞体病的聚合酶链式反应(PCR)诊断方法的研究。
- The sensitive and specific PCR method for detection E. granulosus from dog faeces was preliminarily developed. 初步建立了灵敏、特异的检测家犬粪便中细粒棘球绦虫的PCR方法。
- PCR method was considered as the gold standard, which showed the highest specificity (100%) and sensitivity (100%). PCR法是检测MRSA的金标准,特异性和灵敏度均为100%25。
- The NDI gene in mtDNA was amplified with regular PCR method, and the PCR product was sequenced after purified. 用PCR扩增人线粒体NDI基因片段,PCR产物经纯化后测序和核苷酸同源性分析。