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- Methods A real time quantitative PCR(RQ-PCR) was performed to measure the bcr/abl~(P190) transcripts in the bone marrow mononuclear cells from 74 CML patients in different phases. 方法应用实时定量聚合酶链反应(RQ-PCR)检测74例CML患者骨髓单个核细胞中的bcr/ablP190转录本。
- Keywords real time quantitative PCR;Roundup Ready soybean;GMO detection; 荧光定量PCR;转基因大豆;转基因检测;
- Fluorescence real- time quantitative PCR 实时荧光定量PCR
- The copy number of the gene in chromosome of strain detected by the real time quantitative PCR 荧光定量PCR检测细菌染色体上基因拷贝数
- Multiplex qualitative PCR and real - time quantitative PCR for detection of genetically modified foods 转基因食品多重定性PCR及实时荧光定量PCR检测方法的研究
- Development and Application of Fluorescence Real - time Quantitative PCR for Detecting Listeria Monocytogenes 荧光实时定量PCR检测单核李斯特菌方法学建立及应用
- Measurement of telomere length of intercapillary cells by real - time quantitative PCR 定量聚合酶链反应对系膜细胞端粒长度的测定研究
- Keywords coinfection;Borrelia burgdorferi;Anaplasma phagocytophilum;Lyme disease;real time quantitative PCR; 混合感染;伯氏螺旋体;嗜吞噬细胞无浆体;莱姆病;实时荧光定量PCR;
- Keywords Co-infection;Borrelia burgdorferi;Anaplasma phagocytophilum;Lyme disease;Real time quantitative PCR; 混合感染;伯氏疏螺旋体;嗜吞噬细胞无浆体;莱姆病;细胞因子;
- Keywords Real Time Quantitative PCR;chronic myelogenous leukemia;imatinib mesylate;BCR-ABL fusion gene;resistance mechanism; 实时定量PCR;慢性髓细胞白血病;伊马替尼;BCR-ABL融合基因;耐药机制;
- Real - time quantitative PCR 实时定量PCR
- real time quantitative PCR 实时荧光定量PCR
- The real- time quantitative PCR 定量PGR
- real- time quantitative PCR 实时定量PCR
- Methods A model of animal experiment,a kit for sexdeter mining region Y (SRY) and a set of standard sample were developed for drawing standard curve by real time fluorescence quantitative PCR. 方法结合动物实验模型,设计性别决定因子Y(SRY)实时荧光定量聚合酶链反应(PCR)检测血细胞嵌合率的方法,建立质控标准品,使用实时荧光定量PCR技术,获得标准曲线。
- real - time quantitative RT - PCR 实时定量RT-PCR
- real time fluorescent quantitative PCR 实时荧光定量PCR
- Fluorescence quantitative PCR combined with RT-PCR technique can detect the DTC in blood of the patients with HCC. RT-PCR联合荧光标记探针杂交法能够检测到肝癌患者血液中播散的肿瘤细胞,并且以肺动脉血中阳性率最高。
- For special aims, multiple PCR, reverse PCR, quantitative PCR, in situ PCR, as well as capillary PCR, were developed. 而适合不同目的的PCR技术也得到了充分的发展,如多重PCR、反转录PCR、定量PCR、原位PCR、PCR突变、毛细管PCR技术等等。
- The expression of RAE-1 and H60 genes was measured by RT-PCR and real-time quantitative PCR. 6) BALB/C小鼠正常肝脏组织能检测到RAE一1低表达。
