Results: Hybridoma cells growed well in 12 spots with DMEM culture.And of the 12 spots,6 spots chromosomes had good shapes and stead numbers.

• 结果:12孔板中加入DMEM培养液的杂交瘤细胞株生长良好,其中6孔板加秋水仙素培养1h,染色体形态最佳,数目稳定。