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- Abstract.In this study, we aimed at validating a rotary cell culture system (RCCS) bioreactor with medium recirculation and external oxygenation, for cartilage tissue engineering. 摘要:此研究中的主要目的在于证实旋转细胞培养系统(RCCS)的生物反应器在软骨组织工程中的实际应用效果。
- In this paper, to use Cytodex-3 as microcarrier, we established Three-dimensional (3D) culture of rat WB-F344 cells with rotary cell culture system (RCCS) to simulate microgravity environment. 本文以大鼠肝干细胞系WB-F344为实验材料,采用RCCS系统模拟微重力效应,并以Cytodex-3为微载体对细胞进行培养(以平面培养的细胞为对照),分析微重力三维培养条件下细胞形态、增殖、细胞内部显微结构及细胞粘附分子表达情况;
- The Rotary Cell Culture System (RCCS) from Synthecon is a new development in bioreactor technology which enables the cultivation of differentiated three dimensional cultures that mimic the structure and function of parental tissues. 凭借其生长和维持聚集物那样的活组织的能力,细胞和组织科学家有条件对新生和外植细胞的生长和相互作用的基本过程进行研究。
- Objective Rotary Cell Culture System (RCCS) &Microcarrier Suspension Culture System is one new technique that connected with microcarrier and bioreactor, now is widely used in tissue engineering. 旋转生物反应器内微载体扩增骨髓间充质干细胞骨组织工程的研究目的微载体细胞培养技术是一种微载体与生物反应器结合的技术,现己广泛应用于组织工程领域。
- The rotary cell culture system, RCCS 本研究应用三维组织细胞旋转培养系统
- Experiment on fibroblast-PGA complexes cultured in rotary cell culture system 旋转系统下三维培养成纤维细胞-PGA复合物的实验研究
- Keywords dental pulp stem cell;inducement and differentiation;3D culture;microcarrier;rotary cell culture system; 牙髓干细胞;诱导分化;三维培养;微载体;旋转细胞培养系统;
- The monitoring to secretory function of vascular endothelial cell experimental study by tissue engineering blood vessel approach in rotary cell culture system 动态旋转系统构建组织工程化血管模型中血管内皮细胞分泌功能监测
- Experimental study on constructing small caliber artery with tissue engineering approach in rotary cell culture system 在动态旋转系统中构建小口径组织工程化血管的实验研究
- Keywords immortalization;skin tissue engineering;rotary cell culture system;microcarrier; 永生化;表皮组织工程;旋转生物反应器;微载体;
- Rotary cell culture system 旋转式细胞培养系统
- The establishment of callus culture system and the suspension cell culture system of Syringa pubescens Turcz. 建立了小叶丁香的愈伤组织培养体系和悬浮细胞培养体系
- This paper reviews the current progress of studies on the application of alginate based tri-dimension cell culture system in bone tissue engineering. 摘要目的综述藻酸盐三维细胞培养系统在骨组织工程中的应用研究进展。
- Objective To investigate the proliferation an d metabolism of human dermal fibrobl asts in fibrin gel-a three-dimensio nal cell culture system. 目的观察人皮肤成纤维细胞在立体培养条件下的生长、增殖及代谢情况。
- This thesis reviewed on corneal epithelial cell culture system,focused on common method of culture corneal epithelial cell in vitro,the choices of medium and carrier,the method of differentiation. 作者从角膜上皮细胞的一般体外培养方法、培养基的选择、载体的选择和鉴别方法等方面对角膜上皮细胞培养体系进行了综述。
- Rotating cell culture system (RCCS) 软旋转培养仪
- Stem cells grew, multiplied and differentiated into brain cells on a new three-dimensional scaffold of tiny protein fragments designed to be more like a liing body than any other cell culture system. 干细胞在新的小蛋白碎片三维框架生长、繁殖和分化成为脑细胞,比在其他细胞培养体系中更好。
- Objective To establish an adult bovine lens epithelial cells culture system, and investigate the growth characteristics of the cells in different culture media and its ultrastructure. 目的建立成年牛眼晶状体上皮细胞的培养方法,研究其在不同培养液中的生长特性并观察其超微结构。
- Sensitive cell culture systems include primary bovine thyroid cells and primary pig, calf or lamb kidney cells. 敏感细胞包括原代牛甲状腺细胞和原代猪、犊牛和羔羊肾细胞,
- Embryonic stem cell culture system ES培养体系