Secondly, in order to select the optimum concentration of VEGF, 1,2 or 5 ng/ml VEGF was supplemented in culture media during IVM, IVFand IVC1 in Exp.

• 二、为了选择最适宜的VEGF添加浓度，根据试验1和2的结果，在IVM、IVF、IVC1过程中，设计1、2、5ng/ml VEGF的添加浓度。