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- In this assay,we studied the possibility of raccoon pox virus (RCN) TK gene as a viral selection marker on Rat-2 cells. 本文在Rat-2(TK~-)细胞上,对浣熊痘病毒TK基因作为病毒筛选标记的作用进行了研究。
- Objective To establish a protein expression system using pyrG gene as a selection marker and Aspergillus niger (A.niger) as a host. 目的 建立以pyrG基因为选择标志 ,以黑曲霉菌为宿主菌的蛋白质表达系统。
- These selection marker systems are potential for variety of application in food fermentations or live vehicles and so on. 双质粒选择标记系统构建的筛选过程新颖独特,为整个选择标记系统的发展开辟了新的途径及思路.
- Abstract:Selective marker gene is usually used to select transformed cells or tissue during gene transfer. 在基因转移过程中,人们常常使用标记基因来筛选转化细胞或组织。
- In plant genetic engineering, selectable marker is needed to distinguish transformant. 在植物基因工程中,需要用到选择标记基因以便筛选转化子。
- In order to increase the safety of transgenic plants, Biologists began to search for biosafe selectable marker. 为了提高转基因植物的安全性,生物学家们开始寻找生物安全的选择标记。
- A new strategy that offers a approach for the elimination of those disadvantages caused by the selectable marker gene is developed. 为了消除这些弊端,一种全新的发展策略即获取无选择标记的转基因植物应运而生。
- food-grade selection marker nisI 食品级选择标记nisI
- There are several unique restriction sites on each selective marker,AhdI, PstI, PvuI sites on the marker of Apr and Psp, EcoRI, NcoI on the marker of Cmr. 该质粒具有Apr和Cmr两个抗性标记,在其氨苄青霉素抗性基因上有AhdI、PstI、PvuI插入位点,在氯霉素抗性基因上有Psp、EcoRI、NcoI插入位点。
- The presence in transgenic plants of antibiotic and herbicide resistant selective marker genes might be an unpredictable hazard to the ecosystem as well as to human health. 摘要转基因植物中的除草剂和抗生素抗性标记基因潜在的生态环境和食用安全性令人担忧。
- As the commercialization of transgenic plants, people are more and more paying close attention to their safety, among which refers to the safety of selectable marker. 随着转基因植物的商品化,人们越来越关注其安全性问题,其中主要涉及选择标记的安全性。
- Selection markers on frame numbers bar and Layer Captions show the actual one frame selection. 选项标记上的数字条和层标题表示当前处于活动的帧.
- Study of selectable markers in genetic engineering of Chlorella sp. 小球藻基因工程选择标记研究。
- Currently used plant transformation systems require selectable marker genes encoding antibiotic or herbicide resistance, along with the gene of interest, to select transformed cells from a large population of mostly untransformed cells. 摘要在目前的植物转化系统中,要求在关注基因或目的基因转入细胞时,同时有标记基因存在。
- The results from RT-PCR indicated the knockdown of mRNA level of hCAP-E and hCAP-C in the co-transfeted Hela cells. We also conducted the co-transfection with the RNAi plasmids RHE and RHC and the selective marker GFP expressing plasmids simultaneously. 进行RT一PCR检测表明人集缩素SMC亚基hCAP一E和hCAP一C的C一末端真核表达质粒在Hela细胞内得到过表达。
- Seed chip GUS assay and herbicide leaf painting and spraying assays were applied to test the gus reporter gene and the herbicide resistant bar selectable marker gene, respectively. 分别采用种子切片GUS染色方法和除草剂涂抹以及喷洒方法检测gus报告基因和抗除草剂bar基因在后代的表达。
- Abstract: To improve the efficiency of targeted gene replacement (TGR), a dual screen (DS) system with gusA gene as negative selective marker (GUS-DS) was developed in Magnaporthe oryzae. 摘 要: 目标基因替换是基因功能研究的重要方法, 在生物工程领域广泛应用。
- That shop has a fine selection of cakes. 那家商店有各种精美的糕点供我们选择。
- That store has a good selection of furniture. 那家店有很多家具可供挑选。
- The selection was weighted against me. 那次选拔对我不利55。