Sepharose 6B was activated by epichlorohydrin in the strong base condition, and then reacted with solution of iminodiacetic sodium.The arms of IDA were conjuncted to the activated Sepharose 6B.

• 以Sepharose 6B为原料，在强碱性条件下用环氧氯丙烷活化，再与亚氨基二乙酸（IDA）的钠盐溶液反应，在活化好的胶颗粒表面接上很多手臂IDA；