T1 seeds were harvested and then used to genetic analysis after selection in the kanamycin cuture. We obtained 4 transgenic lines with segregation of 3:1,and confirmed 2 lines expressing CED-9 by RT-PCR.

• 转基因烟草植株后代遗传分析，表明其中4个株系的转基因后代小苗Kan抗性呈3：1的分离比，并用RT-PCR检测到2个株系的烟草后代有CED-9基因的表达。