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- TaqMan Primer and Probe design 设计引物,原则同择
- Main reagent : dilution fluid, hemolysis fluid, Bcr/Abl(P230) , primer and probe of GAPDH gene, primer of DCC gene, RNA extracting fluid, cDNA kit. 主要试剂:血液稀释液、溶血液、Bcr/Abl(P230)和内参GAPDH基因引物及探针、DCC基因引物、RNA提取液、cDNA合成试剂盒,乙酸钠-柠檬酸缓冲液(pH:4.;3)为流动相。
- The results showed that the synthesis and purification system of primers and probes was high efficient and laid the foundation of the high performance of RTF-PCR. 实验结果进一步证明了我们建立的探针合成、纯化体系的高效率和高准确性,为RTF-PCR性能的高效发挥打下了基础。
- FQ-PCR assay for PPV detection establishing According to the published VP2 segment of PPV sequences of PPV in GenBank, a pair of primers and a TaqMan probe were designed. PPV 荧光定量PCR检测方法的建立 根据GenBank中的猪细小病毒基因保守序列设计了用于FQ-PCR的一对引物和一条TaqMan探针。
- So conservative primers and probes were designed according to the conservative sequences of four viruses- CSFV, PRRSV, PRV and PPV which could induce reproductive troubles of pigs. 因此,本研究设计了多科探针和引物,初步建立了双重时荧光PCR技术,检测包括CSFV在内的4种可引起繁殖障碍的病毒,即猪瘟病毒(CSFV),猪繁殖与呼吸综合征病毒(PRRSV),猪伪狂犬病寿(PRV)、猪细小病毒(PPV)。
- With a pair of primers and the E. 通过一对自行设计的引物;以E.
- Besides,using rusted surface primer and functional coating is a good measure. 认为采用锈面底漆与多功能环氧涂料是较好办法。
- The working principle of the new viscosimeter with quartz crystal is discussed in this paper.The method for probe design and the constitution of signal sampling and processing system are presented. 本文讨论了新型石英晶体粘度测量仪的工作原理,介绍了探头设计的方法以及信号采集和处理系统组成。
- From dragonne>remember:dont attack my base and probe before u kill my dragoon. (按约定:你在杀掉龙骑士之前不能动我的基地和农民。)
- The formulation and process of epoxy primer and polyester polyurethane top finish in this system were introduced. 介绍了该涂料体系的环氧底漆和聚酯聚氨酯面漆的配方和生产工艺。
- I'm pricked and probed by what feels like knives, not needles. 我感觉就像有一把把刀而不是针在刺我的胳膊。
- Leverage upper cover and probe plate for PCB easy placing and picking. 省力式上盖及针板,待测板取放容易。
- A cylindrical,usually metal casing containing the primer and charge of ammunition for firearms. 弹药筒一种盛装火器的雷管和弹药的圆柱形金属筒。
- UEW-Y is a kind of double coatings enameled copper round wire, thermal level 130, with polyurethane resin as primer and polyamide resin as finish. 是以聚氨酯树脂为底层,聚氨酯树脂为表层涂制而成的,热级为130的双涂层漆包铜圆线。
- A cylindrical, usually metal casing containing the primer and charge of ammunition for firearms. 弹药筒一种盛装火器的雷管和弹药的圆柱形金属筒
- UEW-E is a kind of double coatings enameled copper round wire, thermal level 155, with polyurethane resin as primer and polyamide resin as finish. 是以聚氨酯树脂为表层涂制而成的,热级为155的双涂层漆包铜圆线。
- A probe that claims to measure either V-series circuits, frame-relay and/or ATM links will most likely provide less data than a probe designed for just one specific media. 声称能测量V系列电路、帧中继/或ATM链路的探头可能不如针对一种特定介质设计的探头提供的数据多。
- EIW-Y is a kind of double coating enameled copper round wire, thermal level 180, with polyesterimid resin as primer and polyamide resin as finish. 是以聚酯亚胺树脂为底层,聚酯胺树脂为涂表层涂制的,热级为180的双涂层漆包铜圆线。
- A probe that claims to measure either V-series circuits,frame-relay and/or ATM links will most likely provide less data than a probe designed for just one specific media. 声称能测量V系列电路、帧中继/或ATM链路的探头可能不如针对一种特定介质设计的探头提供的数据多。
- We add the 5'AOX1 primer and 3'AOX1 primer amplify pPIC9K and alpha-factor signal peptide sequence was obtained. 考虑到克隆方便,在序列5’端和3’端分别引入BamHI和EcoRI 位点,并在两个酶切位点的外侧分别加上保护碱基。