The DNA encoding VP 2 structural protein was amplified by PCR and recombined into T vector and sequenced Compare gene and amino acid squence of VP 2 of MDVP_Q with that of GPV and MDPV which were registered in the genbank.

• 应用PCR技术扩增了MDPV_Q株的VP2 蛋白基因片段。 将扩增后的VP2 基因重组到pMD18_T质粒载体上 ,并对插入片段进行序列测定。