The Grb-ast8 concatemer was cloned into pET22b expression vector. The result of sequencing confirmed the reading frame of the recombinant plasrnid was correct. Then, the recombinant plasmid was transformed into expression host E.

• 将Grb-ast8串联体基因克隆进大肠杆菌pET22b表达载体，测序表明读码框架正确后，将重组子导入表达宿主菌E.