The L ScFv genes were inserted into the eukaryotic fusion protein expression vector pEGFP N3 and transfected into COS 7 cells to express respectively.
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- 将所构建的单链抗体基因克隆入绿色荧光蛋白 (GFP)融合表达载体pEGFP N3,并转染COS 7细胞进行表达。