The antibacterial active components were also purified by another column, reversed phase chromatography column (FPLC RESOURCE RPC 3mL), special peaks were found from the sample of infected insect.

• 5、弱阳离子柱CM-Sepharose Fast Flow层析后有抗菌活性的组分再经反相柱FPLC RESOURCE RPC 3mL层析柱层析，同样也得到特异的峰，表明FPLC RESOURCE RPC 3mL层析柱可以考虑作为第三步分离纯化的层析柱。