The asymmetric reduction of ethyl 4-chloroacetoacetate(COBE) to ethyl(R)-(+)-4-chloro-3-hydroxybutyrate((R)-CHBE) by the recombinant cells in an aqueous phase was investigated.

• 将重组细胞用于催化4-氯乙酰乙酸乙酯不对称还原；合成出具有光学活性的(R)-(+)-4-氯-3-羟基丁酸乙酯.