The best combination of isolating Protoplasts of Sauru- rus chinensis was the enzyme mixture containing 1.5% Onozuka R-10,0.1%Macerozyme R-10 0.7mol/L mannitol and 10 hours of treating time.

• 4通过L9(3~4)和L16(4~5)的正交设计，对酶溶液最佳配方及影响原生质体融合的因素进行了研究，结果表明：分离三白草原生质体酶溶液的组成以1.；5%25的纤维素酶、0