The best expansion conditions for MSC in vitro was 1 l04/ml cell density, 10%FBS, 10ng/ml EGF and 10ng/ml PDGF-BB. After the induction of RA, MSC differentiated into neurons without dopaminergic neuron phenotype.

• 以接种密度为1×10~4个/ml、加入10%25胎牛血清、10ng/ml EGF和10ng/ml PDGF-BB的培养条件最有利于MSC的增殖；