The cell distribution in dermal substitutes from stirred culture system was more uniform than static culture,as well as that with increase of stir speeds in spinner flask.

• 静态培养的细胞-材料复合物内部细胞稀少,且分布不均匀,转瓶培养的细胞-材料复合物在材料表面和内部细胞密度都有所提高,分布情况也得到改善,且80r/min转瓶培养的组织其细胞密度和分布均优于10r/min和40r/min转瓶培养。