The coding sequence of abrin a A chain (ABRaA) gene was obtained by RT PCR and cloned into the expression vector pET28b.

• 用RT PCR方法克隆了相思豆毒蛋白 aA链 (ABRaA)的cDNA编码序列 ,并将其重组到原核表达质粒 pET2 8b中。