The conjugation rate, high molecular conjugate content of and the antibody titer induced with pneumococcus type 1 conjugate vaccine prepared by cyanogen bromide activation were significantly higher than that by amine reduction.

• 采用溴化氰活化法制备的结合物，较胺还原法具有较高的结合率及高分子结合物含量，免疫小鼠产生的抗体效价较胺还原法明显提高。