The constructed pDC316-LMP2 was co-transfected with adenovirus backbone pBHG-fiber5/35 into 293 cells to establish the recombinant adenovirus Ad5F35-LMP2, which was confirmed by PCR and indirect immune-fluorescence test.
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美
- pDC3l6-LMP2与腺病毒骨架pBHG-fiber5/35共转染293细胞构建重组腺病毒Ad5F35-LMP2,PCR及间接免疫荧光进行鉴定。