The effects of nitrogen,glucose, pH,temperature and time of fermentation on chitinase secretion were investigated in 250 mL Erlenmeyer flasks.

• 在250 mL摇瓶中研究了氮、葡萄糖浓度、pH值、培养温度、几丁质类型和培养时间对CCB-1生长及产酶的影响.