The expression of IL-12 protein and STAT4 (signal transducer and act ivator of transcription, STAT) protein in liver grafts was detected by using Wes tern-blotting. DNA binding activity of STAT4 was measured by EMSA.

• 分别采用Western blot及EMSA检测肝移植后 0h和4d移植肝IL 12蛋白表达、STAT4蛋白表达及其DNA结合活性 ;