The expressions of ESM-1 and ERK (external-signal regulated kinase), and the phosphorylation of ERK were detected by immuocytochemistry.

• 观察ECV304形态,用MTT法对ECV304增殖情况进行分析,用免疫细胞化学方法观察ESM-1、胞外信号调节激酶(external-signal regulated kinase, ERK)在ECV304中的表达情况,以及ERK的磷酸化情况。