The extracellular recordings have got from the experiments of that perfuse a brain slice of bufo melanostictus optitectus(OT)in vitro.

• 采用脑薄片技术，用金属微电极对离体蟾蜍视顶盖简称（OT）进行细胞外记录，观察视顶盖OT神经元的自发放电特点及几种神经递质的影响。