The extracts were eluted by 70%,80%,90%ethanol solutions in order in silica gel column chromatography and the refined flovonoids solution was obtained.

• [方法]采用乙醇回流法提取云南13种蕨类植物根状茎(根)及叶中的总黄酮,经硅胶柱层析,依次用浓度70%25、80%25、90%25的乙醇溶液洗脱,得到精制黄酮溶液,利用紫外光分光光度法测定总黄酮的含量。